戊型肝炎是主要的病毒性肝炎之一,国内外尚无疫苗。据估计全球有三分之一人口曾感染过戊型肝炎病毒。戊型肝炎症状与甲型肝炎类似,但病死率更高,症状更重,对孕妇、老年人和慢性肝病患者的危害尤烈,孕妇病死率高达20%。我国是戊型肝炎高流行区,近年来发病率持续上升。 近期,由国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学)、北京万泰生物药业股份有限公司、厦门万泰沧海生物技术有限公司等单位联合研制的戊型肝炎疫苗取得重大突破,三期临床研究证实戊肝疫苗安全有效,等待药监部门批准后即可迅速上市。相关研究成果近日在国际著名医学刊物《柳叶刀》上发表,并得到了美国疾病预防控制中心肝炎室主任Scott D Holmberg博士的高度评价,标志着我国在戊型肝炎疫苗研制方面已达到世界领先水平。 我国研制的戊型肝炎疫苗,迄今已取得4项国内外授权发明专利,是我国少见的高度原创性生物药物之一,国家食品药品监督管理局将其纳入特殊审评程序,于今年8月19号召开了审批咨询会议。目前,国际主要疫苗跨国企业瑞士诺华公司、美国默克公司、法国赛诺菲-巴斯德公司、英国葛兰素史克公司均对合作开发该疫苗的国际市场表现出浓厚兴趣。
… Wang, H Zhang, N Xia, G Peng, HY … - Journal of medical …, 2002 - interscience.wiley.com You-Chun Wang,1,2 Hua-yuan Zhang,1 Ning-shao Xia,3 Geng Peng,4 Hai-Yun Lan,1 Hui Zhuang,5 Yong-Hong Zhu,5 Shao-Wei Li,3 Ke-Gong Tian,6 Wen-Jie Gu,1 Jing-Xiang Lin,1 Xing Wu,1 He-Min Li,1 and Tim J. Harrison2* 1National Institute for the Control of ... Cited by 122 - Related articles - BL Direct - All 6 versions
jbc.org[HTML]… , Y Gu, RS Liu, J Lin, YX Chen, MH Ng, NS Xia - Journal of Biological …, 2005 - ASBMB The hepatitis E virus (HEV) capsid consists of a single structural protein, a portion of which is engaged in isosahedral contact to form a basal shell, and another portion in dimeric contact to form the homodimers protruding from the shell. Previous studies revealed that ... Cited by 28 - Related articles - All 6 versions
NS Xia, WX Luo, J Zhang, XY Xie, HJ Yang, SW Li, … - Marine …, 2002 - Springer Abstract: Studies of the bioluminescent mechanisms of jellyfish have been mainly confined to one species, Aequorea victoria. We describe the luminescent system of another species, Aequorea macrodactyla, which is commonly found in the warmer waters on the coastal region of ... Cited by 18 - Related articles - BL Direct - All 3 versions
… -shun, LI Shao-wei, Z Jun, XIA Ning-shao - Chinese Journal of …, 2005 - en.cnki.com.cn Genomic sequences of genotypes I, II, III and IV of hepatitis E virus were aligned and the conserved region was selected for designing a set of reverse-transcription polymerase chain reaction (RT-PCR) primers, which were denominated as E primers. The sensitivity of E primers ... Cited by 10 - Related articles - Cached - All 2 versions
… Ming-hui, M Ji, J ZHANG, XIA Ning-shao - Chinese Journal of …, 2006 - en.cnki.com.cn We have previously expressed a recombinant hepatitis E virus capsid protein p239(ORF2,aa368-606) which showed good immunogenicity and reactivity,and has been confirmed to have a simulated structure compared with native viruses. In the present study,p239 was ... Cited by 4 - Related articles - Cached
XIE Xiao-Yan, XIA Ning-Shao - Letters in Biotechnology, 2001 - en.cnki.com.cn The phenomenon of fluorescence resonance energy transfer(FRET) can be utilized to evaluate or to detect the distance between macromolecules.Recently,many new material and methods have been introduced into the use of this phenomenon,which has facilicated the ... Cited by 3 - Related articles - Cached
… -lian, MA Ying, W ZHENG, XIA Ning-shao - Letters in …, 2002 - en.cnki.com.cn Half-embryo was used as the acceptor of peanut transformation for the first time.Half-embryo co-culture with Agrobacterium tumefaciens strain EHA105(including plasmid p1301HBsAg)for five days.Through hygromycin selection,transformed peanuts were obtained.The ... Cited by 3 - Related articles - Cached
… Yun, SY Chun, ZZ Zheng, RLK Ho, ECW Chak, NS Xia, … - Apoptosis, 2007 - Springer Abstract BH3-only protein Bim is a critical regulator of apoptosis and plays an essential role in mammalian development, but the characterization of Bim and its iso- forms in hepatocellular carcinoma (HCC) has not been studied before. Here we investigated the expression, dis- ... Cited by 3 - Related articles - BL Direct - All 6 versions
… -yuan, T Ming, J ZHANG, XIA Ning-shao - Chinese Journal of …, 2007 - en.cnki.com.cn By using Western blot and immunofluorescence assays,the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature.Pre-mixture of wild-type HEV with p239 ... Cited by 2 - Related articles - Cached
… SUN Yuan-yuan, J ZHANG, XIA Ning-shao - Chinese Journal of …, 2006 - en.cnki.com.cn For further understanding the infection mechanism and pathogenesis of hepatitis E virus(HEV),we screened the protein interacting with HEV virus-like particle(p239) extracted from HepG2 cells by affinity chromatography,two-dimensional electrophoresis and mass ... Cited by 2 - Related articles - Cached
… -tang, K Wei-min, Z Jun, XIA Ning-shao - Chinese Journal of …, 2004 - en.cnki.com.cn Objective The status of hepatitis E virus infection was under investigation in a general population based on a novel antibody detection assay.Methods Anti-HEV IgG ELISA based on polymerized recombinant HEV antigen (E2-IgG ELISA) was used.Results The prevalence of HEV ... Cited by 2 - Related articles - Cached - All 2 versions
… , LIN Jian, LIUR Yi_Xin, W Ying_Bin, XIA … - Chinese Journal of …, 2004 - en.cnki.com.cn ... 3, YAN Qiang,DU Hai-lian,WANG Ying-bin,GE Sheng-xiang,HUANG Shou-jie,JIANG Lang,ZHANG Jun,XIANing-shao(National Institute of Diagnostics and Vaccine Development in Infectious Disease,Xiamen University,Xiamen 361005,China);Comparison of two diagnostic ... Cited by 6 - Related articles - Cached
[CITATION] Department of Clinical Laboratory, Beijing Railway General Hospital, Beijing 100038, China; Serological characteristics of a hepatitis E outbreak [J]
… -ying, C Yan, P Geng, S Wei, Z Man, XIA Ning-shao - Chinese Journal of …, 2003 Cited by 2 - Related articles
… , H Shou-jie, J Lang, Z Jun, XIA Ning-shao - Chinese Journal of …, 2008 - en.cnki.com.cn The sensitivity and specificity of two diagnostic reagent to detect the presence of anti-hepatitis E virus IgG antibodies(E2-IgG and X-IgG) were tested in 460 serum samples taken from population undergoing an epidemiological investigation of Jiangsu province in 2006 and 72 serum ... Cited by 2 - Related articles - Cached
HE Zhiqiang, G Shengxiang, XIA … - Chinese Journal of …, 2002 - en.cnki.com.cn ... 1, WU Ting,OU Shan-hai,CHENG Tong,HE Shui-zhen,WU Xiao-lu,ZHENG Zhou,ZHANG Jun,XIANing-shao~*(The Research Center for Medical Molecular Virology of Fujian Province,Xiamen University,Xiamen 361005,China);Immune Response Induced by Recombinant ... Cited by 5 - Related articles - Cached - BL Direct
QU Yong-gang, ZHU Guang-ze, JIN … - Chinese Journal of …, 2008 - en.cnki.com.cn ... 7, YAN Qiang,DU Hai-lian,WANG Ying-bin,GE Sheng-xiang,HUANG Shou-jie,JIANG Lang,ZHANG Jun,XIANing-shao(National Institute of Diagnostics and Vaccine Development in Infectious Disease,Xiamen University,Xiamen 361005,China);Comparison of two diagnostic ... Cited by 4 - Related articles - Cached
QIN Ai_jian, S Hong_xia, Q Kun, LIU … - Chinese Journal of …, 2003 - en.cnki.com.cn ... 5, CHEN Yi-xin~1,LUO Hai-feng~1,GE Sheng-xiang~1,GUO Yong-li~1,PENG Geng~2, WANG Jia~3,CHEN Hong-lin~(3,4),ZHANG Jun~1,GUAN Yi~(3,4),XIANing-shao~1(1.The Key Laboratory of Education Minister for Cell Biology and Tumor Cell Engineering of Xiamen ... Cited by 6 - Related articles - Cached
… Yuan-yuan, T Ming, Z Jun, XIA Ning-shao - Journal of Microbes …, 2007 - en.cnki.com.cn Objective To further investigate the interaction between recombinant hepatitis E virus (HEV) capsid protein p239 and Grp78/Bip and the role of Grp78/Bip in HEV penetration.Methods We utilized pull-down, immunoprecipitation and antibody blocking assays to examine the interaction ... Cited by 1 - Related articles - Cached - All 2 versions