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佛罗里达大学研究人员发现“短吻鄂”细菌可提升纤维素乙醇生产

已有 6096 次阅读 2009-8-18 08:25 |个人分类:生物质能|系统分类:科普集锦| 生物燃料, 纤维素, 生物质能

 
王应宽编译
2009-08-17
UTC-6 CST
UMN, St Paul
 
 
佛罗里达大学研究人员发现“短吻鄂”细菌
可提升纤维素乙醇生产
 
据位于甘斯韦尔的佛罗里达大学消息(July 27, 2009),该校的研究人员发现生长在“短吻鳄”树中的细菌有助于改善生产木质纤维素乙醇的工艺,将有助于解决国家的能源危机。(注:佛罗里达大学橄榄球队取名短吻鳄队- the Gators
 
纤维素乙醇燃料的生产源于经常被丢弃的植物废弃物。典型的工艺是采用转基因工程的细菌或复杂的化学反应分解植物细胞壁中的复杂化合物,来生产单糖分子化合物,再发酵生产燃料级乙醇。
 
圣地亚国家实验室(Sandia National Laboratories2月份的报告预测,如果能降低生产成本,到2030年纤维素乙醇将可取代全美国汽油消耗量的30%。而降低成本的重要途经就是使生产更高效。
 
纤维素乙醇生产中效率低的原因很大程度在于在反应之初必须要用加热和加酸的办法预处理植物原料以分解植物细胞壁中的一些成分。佛罗里达大学食品与农业科学研究所的研究组在应用与环境微生物学学报(Journal Applied and Environmental Microbiology)报道称,有一种命名为JDR-2的朽木细菌(Paenibacillus Sp.)具有分解半纤维素的特殊能力。这种能力有助于改进预处理工艺步骤以便低成本高效率的生产乙醇。
 
通过工程改良细菌,像类芽孢杆菌JDR-2那样分解半纤维素,纤维素乙醇生产工艺可以大大简化。研究组几年前就筛选出了这种细菌,并被用于枫香树进行分解试验。目前研究组已经绘制出JDR-2的基因组图谱,预计年内将把JDR-2的纤维素分解能力转基因到细菌,用于生产乙醇。届时将设计高效的工艺以便用木材、农业废弃物和其他能源作物生产燃料乙醇。
 
 
资料来源:
 
UF team finds ‘alligator tree’ bacteria might improve cellulosic ethanol production
 
UF team finds ‘alligator tree’ bacteria might improve cellulosic ethanol production
 
Filed under Agriculture, Environment, Florida, Research on Monday, July 27, 2009.
GAINESVILLE, Fla. — Most would identify the tree by its often troublesome, spiky “gumballs,” but what many call the sweetgum tree also goes by another name, thanks to its distinctive, reptilian bark: the alligator tree.
 
So it may be fitting that researchers from the University of Florida, home of the Gators, have found that bacteria growing in its wood may improve the process of making the fuel that might help solve the nation’s energy crisis.
 
Cellulosic ethanol fuel is derived from plant material often thrown away as trash. Typically, the processes use genetically engineered bacteria or tricky chemical reactions to break down complex compounds in plant cell walls to produce simple sugar molecules that can be fermented into fuel-grade alcohol.
 
A February report by the Sandia National Laboratories predicted that cellulosic ethanol could replace 30 percent of the nation’s gasoline by 2030 if the price can be brought down. A big part of reducing the price is making production more efficient.
 
Much of the inefficiency in cellulosic ethanol production lies in the fact that it must be given a head start by cooking the plant material with heat and acids to break down some of the components in the plant cell walls.
 
As the team from UF’s Institute of Food and Agricultural Sciences reported in the July issue of the journal Applied and Environmental Microbiology, a strain of the wood-decaying Paenibacillus sp. bacteria named JDR-2 has a knack for breaking down and digesting one of these components, hemicelluloses.
 
That knack could help modify preprocessing steps for cost-effective production of ethanol.
 
“The acids, the heating — all of these steps you have to take beforehand are expensive, require a lot of work and, let’s face it, no one wants to work with sulfuric acid on that scale if you don’t have to,” said James Preston, the team leader and a professor in UF’s microbiology and cell science department.
 
“By engineering the bacteria already being used to produce ethanol to also process hemicelluloses the way this Paenibacillus does, you should be able to significantly simplify the process.”
 
Preston came across the bacteria a few years ago, as he was using decaying sweetgum trees to grow shiitake mushrooms on his tree farm in Micanopy, Fla. After studying the unusually uniform composition of the decaying wood, he and his colleagues went on to study the genetics of one of the bacteria digesting that wood.
 
The team has now mapped JDR-2’s genome, and Preston expects that, within the year, they will transfer genes behind JDR-2’s abilities to bacteria used to produce ethanol. This would be followed by the design of processes for the cost-effective production of ethanol from wood, agricultural residues and other potential energy crops.
 
Credits
Writer
Stu Hutson, stu@ufl.edu, 352-392-0400
Source
James Preston, jpreston@ufl.edu, 352-392-5923
 
 


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