日本学者随后证明呼吸2%氢可治疗肝缺血再灌注损伤(2007)

实验采取的方法十分简单, 制备肝缺血动物模型, 呼吸氢后,取组织观察HE染色确定能改善组织损伤, 测定血液中肝功能相关酶(临床常规检测手段)确定肝功能改善, 采用MDA(氧化损伤产物)确定具有抗氧化作用. 发表在 BBRC上. 尽管文章不是非常全. 如此简单的研究能发表在这个杂志上, 强烈说明这个思路的新颖性. 我看到 Nature的文章的感觉是吃惊, 看到这个文章的感觉是惊叹. 这两个文章直接引起我们强烈的兴趣: 氢真的有这样好的效果吗? 为什么? 抗氧化的研究已经有很多.氢到底神奇在什么地方?

我们随后进行了跟踪研究, 结果证明确实十分意外:效果太好了!!!!!!

Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress.

[My paper] Kei-Ichi Fukuda, Sadamitsu Asoh, Masahiro Ishikawa, Yasuhiro Yamamoto, Ikuroh Ohsawa, Shigeo Ohta
We have recently showed that molecular hydrogen has great potential for selectively reducing cytotoxic reactive oxygen species, such as hydroxyl radicals, and that inhalation of hydrogen gas decreases cerebral infarction volume by reducing oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K.-I. Katsura, Y. Katayama, S. Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med., 13 (2007) 688-694]. Here we show that the inhalation of hydrogen gas is applicable for hepatic injury caused by ischemia/reperfusion, using mice. The portal triad to the left lobe and the left middle lobe of the liver were completely occluded for 90min, followed by reperfusion for 180min. Inhalation of hydrogen gas (1-4%) during the last 190min suppressed hepatic cell death, and reduced levels of serum alanine aminotransferase and hepatic malondialdehyde. In contrast, helium gas showed no protective effect, suggesting that the protective effect by hydrogen gas is specific. Thus, we propose that inhalation of hydrogen gas is a widely applicable method to reduce oxidative stress.